Histology and Imaging Core (HIC) - University of Washington

Department of Comparative Medicine - HIC - CPP - GNAC - BSL3/ABSL3 - IVS - TRP - UWMCC - UW PRO

COVID-19 Update

The Histology and Imaging Core staff are currently working remotely to the fullest extent possible and are not staffing our facility in person. If you are interested in any of our
services or have a question about your samples please contact Brian Johnson for more information.

brianj18@uw.edu.

Under the mandated “Stay Home, Stay Healthy proclamation the Office of Research has adopted criteria for certain “in-person”
research to continue. To see if your research meets these criteria please visit the Office Of Research Website, https://www.washington.edu/research/

We are following these guidelines and hope to be back in the lab after April 8^th Please keep in mind this is a fluid situation and this date may be extended.

We look forward to working with you again soon.

Brian Johnson

What We Can Provide

UW Histology and Imaging We provide efficient, high-quality solutions for your research needs including:

Immunohistochemistry
Image analysis
Quantitative microscopy
Histology
Luminex cell based multiplex assay
Comparative pathology consultation

We employ extensive experience, expertise and state of the art high-throughput instrumentation across all platforms in our facility. This core is open to all investigators, including; UW and their affiliates, commercial and not-for-profit biotechnology companies, and other academic institutions. Through our partnership with the Comparative Pathology Program (CPP) we have access to experienced comparative pathologists who are available for research consultation and collaboration.

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Optimized Antibodies

The HIC utilizes automated immunohistochemistry stainers which provide efficiency and consistency as well as standardization of protocols across studies of all sizes.

Please review the list of over 100 antibodies that have been optimized in our core. We are now pleased to offer additional high throughput automated IHC staining on our new Leica Bond Rx platform. Please contact Brian Johnson for more information

Invitrogen Multiplex (Luminex) Panel Configurator & Selection Tool

Easily and conveniently design your own custom Invitrogen multiplex immunoassay for protein quantitation detection using Luminex® technology. Select a species, panel type, and any number of available targets, and have the panel built and validated for you. This tool features our Invitrogen ProcartaPlex Assays (formerly eBioscience) and the Invitrogen legacy Luminex Assays.

For questions about custom multiplex assays and services, including ongoing support for legacy Luminex assay products, contact AFFYTech_Support@thermofisher.com.

URL: www.thermofisher.com/findluminex

Contact Brian Johnson (206-685-6517) for more information.

Equipment

State of the art Histology, Immunohistochemistry and Digital Microscopy technologies are utilized at the HIC. Click here for a list of equipment.

Work Order

Work Orders may be submitted via online forms. DRC and CFTRC investigators, please note special instructions at the top of the form before submitting your work order. General Histology Guidelines and Submission Guidelines are also available.

Contact Us

We encourage anyone interested in using our services to consult with us during the planning stages of your project to help ensure the best possible outcome.

For more information on the resources available, see the HIC Resource Statement.

Please stop by the Histology and Imaging Core in Room N310 or call to familiarize yourself with our resources, to discuss your histology and imaging needs, or to schedule deliveries to our facility at SLU. Brian Johnson can be contacted by phone (206) 685-6517 or e-mail.