Optimized Antibodies

The HIC utilizes automated immunohistochemistry stainers which provide efficiency and consistency as well as standardization of protocols across studies of all sizes. Please review the list of over 100 antibodies that have been optimized in our core.

Please feel free to consult with HIC staff to discuss antibody selection, fixation techniques and control tissue for any IHC project. Some antibodies are simply not effective for immunohistochemistry techniques and the HIC staff can help you determine the best marker for your study needs. We are constantly optimizing new (to our core) antibodies so please contact Brian Johnson if an antibody you are interested in is not on our optimized list.

Antibody	Catalog Number	Company	Raised in:	Ractive to:	Datasheet link
AF-488	A-11001	Life Technologies	Goat	Ms	http://products.invitrogen.com/ivgn/product/A11001
AF-546	A-11010	Life Technologies	Goat	Rb	http://products.invitrogen.com/ivgn/product/A11010?ICID=search-a11010
AF-594	A11062	Invitrogen	Rabbit	Ms	http://products.invitrogen.com/ivgn/product/A11062?ICID=search-a11062
Annexin A2	60051-1-Ig	ProteinTech	Mouse	Hu, Ms
ANP	ab91250	Abcam	Rabbit	Hu, Ms, Mk	http://www.abcam.com/anp-antibody-n-terminal-ab91250.html
APOM	LS-B5901	Lifespan Bioscience	Goat	Ms, Hu	http://www.lsbio.com/Documents/PDF/Antibodies/141308
Arginase 1	sc-20150	Santa Cruz Biotechnology	Rabbit	Hu, Ms, Rt	http://datasheets.scbt.com/sc-20150.pdf
Armenian Hamster 2°	127-035-160	Jackson ImmnunoResearch	Goat	A. Hamster	https://www.jacksonimmuno.com/catalog/quickfind
B-Catenin	ab16051	Abcam	Rabbit	Ms, Rt, Hu
BrdU	ab6326	Abcam	Rat	Ms	http://www.abcam.com/BrdU-antibody-BU1-75-ICR1-ab6326.html
C-Fos	PC38	Calbiochem	Rabbit	Hu,Ms,Rt	http://www.ebiotrade.com/buyf/productsf/calbiochem/pc38.pdf
Calbindin	300	Swant	Mouse	Rb	http://www.labome.com/product/SWant/300.html
Calbindin	CB38	Swant	Rabbit	Mu, Ms, Rt, Mk	http://1degreebio.org/reagents/product/504930/?qid=0
CAMP	LS-C94098	Lifespan Bioscience	Rabbit	Ms	http://www.lsbio.com/Documents/PDF/Antibodies/95319
CC3	CP229B	BioCare Medical	Rabbit	Hu, Ms	http://biocare.net/wp-content/uploads/229.pdf
CCR6	MAB195	R&D Systems	Mouse	Hu	http://www.rndsystems.com/Products/MAB195
CD3	MCA1477	AbD Serotec	Rat	Hu, Ms	http://www.abdserotec.com/catalog/datasheet-MCA1477.html
CD3	NCL-L-CD3-565	Leica	Mouse	Hu	http://www.leicabiosystems.com/ihc-ish/novocastra-reagents/primary-antibodies/details/product/cd3-1/
CD4	550278	BD Pharmingen	Rat	Ms	http://www.bdbiosciences.com/ptProduct.jsp?prodId=18261&key=550278&param=search&mterms=true&from=dTable
CD4	VP-C319	Vector	Mouse	Hu	http://www.vectorlabs.com/data/protocols/VPC319.pdf
CD4	14-9766-80	eBioscience	Rat	Ms	http://us.ebioscience.com/mouse-cd4-antibody-purified-4sm95.htm
CD8	ab4055	Abcam	Rabbit	Hu	http://www.abcam.com/cd8-antibody-ab4055.html
CD8a	550281	BD Pharmingen	Rat	Ms	http://www.bdbiosciences.com/ptProduct.jsp?prodId=18283&key=550281&param=search&mterms=true&from=dTable
CD8A	14-0808-80	eBioscience	Rat	Ms	http://us.ebioscience.com/mouse-cd8a-antibody-purified-4sm15.htm
CD8	NCL-L-CD8-295	Leica	Ms	Hu	http://www.leicabiosystems.com/ihc-ish-fish/immunohistochemistry-ihc-antibodies-novocastra-reagents/primary-antibodies/details/product/cd8/
CD11c	bs-2508R	Bioss Antibodies	Rabbit	Hu	http://biossusa.com/store/bs-2508r.html
CD14	NCL-L-CD14-223	Leica	Mouse	Hu	http://www.leicabiosystems.com/ihc-ish/novocastra-reagents/primary-antibodies/details/product/cd14/
CD20	NCL-L-CD20-L26	Leica	Mouse	Hu	http://www.leicabiosystems.com/ihc-ish/novocastra-reagents/primary-antibodies/details/product/cd20/
CD31	ab56299	Abcam	Rat	Ms	http://www.abcam.com/CD31-antibody-RM0032-1D12-ab56299.htm
CD31	PA0250	Leica	Mouse	Hu	http://www.leica-microsystems.com/fileadmin/img_uploads/novocastra_reagents/Novocastra_datasheets/pa0250.pdf
CD31	DIA-310	Dianova	Rat	Ms	http://www.dianova.com/en/products/product-highlights/murine-cd31-clone-sz31-formalin-fixed-paraffin-embedded-tissues/
CD45R	557390	BD Pharmingen	Rat	Ms	http://www.bdbiosciences.com/ptProduct.jsp?prodId=881&key=557390&param=search&mterms=true
CD45R	M0701	Dako	Mouse	Hu	http://www.dako.com/us/ar38/p103020/prod_products.htm
CD66b	555723	BD Pharmingen	Mouse	Hu	http://www.bdbiosciences.com/external_files/Doc_Recon_2.0/pm/tds/555723.pdf
CD68	MCA1957	AbD Serotec	Rat	Ms	http://www.abdserotec.com/CodePages/Datasheet.aspx?ProductCode=MCA1957
CD68	PA0273	Leica	Mouse	Hu	http://www.leica-microsystems.com/products/total-histology/novocastra-reagents/primary-antibodies/details/product/cd68/
CD138 (Syndecan-1)	553712	BD Pharmingen	Rat	Ms	http://www.bdbiosciences.com/ptProduct.jsp?prodId=1716&key=553712&param=search&mterms=true&from=dTable
CD163	NCL-L-CD163	Leica	Mouse	Hu	http://www.leicabiosystems.com/fileadmin/img_uploads/novocastra_reagents/Novocastra_datasheets/cd163-l-u.pdf
Claudin-3	PA5-16867	ThermoScientific	Rabbit	Hu	http://www.pierce-antibodies.com/Claudin-3-antibody-Polyclonal--PA516867.html
Cleaved Notch 1	4147	Cell Signaling	Rabbit	Hu, Ms, Rt	http://www.cellsignal.com/products/primary-antibodies/cleaved-notch1-val1744-d3b8-rabbit-mab/4147
CPA1	HPA008689	Sigma	Rabbit	Hu	http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=HPA008689\|SIGMA&N5=SEARCH_CONCAT_PNO\|BRAND_KEY&F=SPEC
Cytokeratin WSS	Z0622	Dako	Rabbit	Cow	http://www.dako.com/us/ar38/p104910/prod_products.htm
Desmin	M0760	Dako	Mouse	Hu	http://www.dako.com/us/ar38/p104050/prod_products.htm
E-Cadherin	3195	Cell Signaling	Rabbit	Ms, Hu	http://www.cellsignal.com/products/primary-antibodies/e-cadherin-24e10-rabbit-mab/3195?hit=productId&Ntt=3195
E. coli	B0357	Dako	Rabbit	Hu	http://www.dako.com/us/ar38/p235383/prod_products.htm
EGFr	28-005	Invitrogen	Mouse	Hu	http://products.invitrogen.com/ivgn/product/280005?ICID=search-280005
ENT1	LSB3385/22621	Lifespan Bioscience	Rabbit	Hu	http://www.lsbio.com/Products/Antibodies/AntibodyDetail.aspx?AntibodyID=106033
ESAT6	sc-57730	Santa Cruz Biotechnology	Mouse	ZF	http://datasheets.scbt.com/sc-57730.pdf
Estrogen Receptor (ERa)	A00010	ScyTek	Mouse	Hu
F4/80	MF48000	Invitrogen	Rat	Ms	http://www.invitrogen.com/site/us/en/home.html
FGF9	ab71395	Abcam	Rabbit	Ms, Hu	http://www.abcam.com/fgf9-antibody-ab71395.html
FLAG	2368	Cell Signaling	Rabbit	Ms	http://www.cellsignal.com/products/primary-antibodies/2368
Flu	B65141G	Meridian Life Science	Goat	Ms	http://meridianlifescience.com/products/results_2.aspx?searchbox=b65141g&page=1&group=0
Foxp3	14-4777-82	eBioscience	Mouse	Hu
Foxp3	ab20034	Abcam	Mouse	Hu	http://www.abcam.com/foxp3-antibody-236ae7-ab20034.html
Foxp3	14-5773	eBioscience	Rat	Ms, Rt	http://us.ebioscience.com/mouse-rat-foxp3-antibody-purified-fjk-16s.htm
Galectin3	PA0238	Leica	Mouse	Hu	http://www.leica-microsystems.com/products/total-histology/novocastra-reagents/primary-antibodies/details/product/galectin-3-1/
GFAP	Z0334	Dako	Rabbit	Ms	www.dako.com/ar38/p235385/prod_products.htm
Glucagon	G2654	Sigma	Mouse	Rt, Hu
Glucagon	G2654	Sigma	Mouse	Ms
Goat Isotype	02-6202	Invitrogen	Goat		http://products.invitrogen.com/ivgn/product/026202?CID=Search-02-6202
Goat Secondary	305-005-045	Jackson ImmnunoResearch	Rabbit	Goat	http://www.jacksonimmuno.com/MERCHANT2/merchant.mv?Screen=PROD&Product_Code=305-005-045
Goat Secondary	G4018	Sigma	Rabbit	Goat	http://www.sigmaaldrich.com/catalog/product/sigma/g4018?lang=en&region=US
GR1	MAB1037	R&D Systems	Rat	Ms	https://www.rndsystems.com/products/mouse-gr-1-ly-6g-antibody-rb6-8c5_mab1037
GranzymeB	ab4059	Abcam	Rabbit	Hu, Ms, Rt	http://www.abcam.com/granzyme-b-antibody-ab4059.html
Guinea Pig Secondary	ab102356	Abcam	Rabbit	GP	http://www.abcam.com/Rabbit-polyclonal-Secondary-Antibody-to-Guinea-pig-IgG-H-L-ab102356.html
H2AX	560447	BD Pharmingen	Mouse	Hu
Her-2	MS-325-P0	ThermoScientific	Mouse	Ms	http://www.thermoscientific.com/ecomm/servlet/productsdetail?productId=11954231&groupType=PRODUCT&searchType=0&storeId=11152&from=search
HIV1 p24	ab19961	Abcam	Goat	Hu	http://www.abcam.com/HIV1-p24-antibody-ab19961.html
HIV1 p24	ab85536	Abcam	Mouse	Hu	http://www.abcam.com/HIV1-p24-antibody-24-2-ab85536.html
HLA-DR	ab92511	Abcam	Rabbit	Hu	http://www.abcam.com/hla-dr-antibody-epr3692-ab92511.html
HLA-DR	M0746	Dako	Mouse	Hu	http://www.dako.com/us/ar38/p104640/prod_products.htm
ICOS	ab105227	Abcam	Rabbit	Hu	http://www.abcam.com/icos-antibody-sp98-ab105227.html
IL-17	AF-317-NA	R&D Systems	Goat	Hu	http://www.rndsystems.com/Products/AF-317-NA
IL-1beta	49-4960	ProSci	Rabbit	Hu	http://www.prosci-inc.com/shop/product.php?productid=33254&cat=0&page=1
Inhibin	PA0110	Leica	Mouse	Hu	http://www.leicabiosystems.com/ihc-ish/novocastra-reagents/primary-antibodies/details/product/inhibin-alpha-1/
iNOS	ab15326	Abcam	Rabbit	Hu	http://www.abcam.com/inos-antibody-prediluted-ab15326.html
iNOS	ABN26	Millipore	Rabbit	Ms, Hu	http://www.emdmillipore.com/US/en/product/Anti-iNOSNOS-II-Antibody%2C-NT,MM_NF-ABN26
Insulin	A0564	Dako	Guinea Pig	Hu	http://www.dako.com/us/ar38/p104840/prod_products.htm
Insulin	I2018	Sigma	Mouse	Rt, Hu, Rb	http://www.sigmaaldrich.com/catalog/product/sigma/i2018?lang=en&region=US
Jak2	3230	Cell Signaling	Rabbit	Ms, Hu, Rt	http://www.cellsignal.com/products/3230.html
Keratin 1	905201	BioLegend	Rabbit	Hu, Rt, Mk
Keratin 14	905301	Biolegend	Rabbit	Hu, Ms, Rt, Dg, Mk	http://www.biolegend.com/keratin-14-polyclonal-antibody-purified-antibody-10953.html
Ki-67	12202	Cell Signaling	Rabbit	Ms	http://www.cellsignal.com/products/primary-antibodies/ki-67-d3b5-rabbit-mab-mouse-preferred-ihc-formulated/12202
Ki-67	M7240	Dako	Mouse	Hu	http://www.dako.com/us/download.pdf?objectid=104980004
Ly6B	MCA771GA	Bio-Rad Antibodies	Rat	Ms	https://www.bio-rad-antibodies.com/mouse-ly-6b-2-alloantigen-antibody-7-4-mca771g.html?f=purified
Mac2	CL8942AP	Cedarlane	Rat	Hu, Ms	http://www.cedarlanelabs.com/Products/Detail/CL8942AP
Mac2	LS-B5661	LS Bio	Rabbit	Hu, Ms, Rt	http://www.lsbio.com/antibodies/anti-mac-2-galectin-3-antibody-internal-ls-b5661-ihc-plus/140267
Malondialdehyde	ab6463	Abcam	Rabbit	Hu	http://www.abcam.com/malondialdehyde-antibody-ab6463.html
MBP	ab7349	Abcam	Rat	Hu, Ms, Rt, Rb	http://www.abcam.com/myelin-basic-protein-antibody-12-oligodendrocyte-marker-ab7349.html
MCT	MS-1216-PO	ThermoScientific	Mouse	Hu	http://www.thermoscientific.com/en/product/mast-cell-tryptase-ab-2-mouse-monoclonal-antibody.html
Mesothelin	28127	IBL America	Rabbit	Ms	http://www.ibl-america.com/pdf/Other_Polyclonal_Antibodies/28127.pdf
Mesothelin	LS-C25601	LS Bio	Mouse	Hu	http://www.lsbio.com/antibodies/anti-msln-antibody-mesothelin-antibody-if-immunofluorescence-ihc-wb-western-ihc-plus-ls-b2618/86124
MHC class II	14-5321	eBioscience	Rat	Ms	http://us.ebioscience.com/mouse-mhc-class-ii-antibody-purified-m5-114152.htm
Mitofusin-1	AP22961PU-N	Acris Antibodies	Goat	Hu, Ms, Rt, Rb	http://us.acris-antibodies.com/antibodies/mitofusin-1-ap22961pu-n.htm
MMP28	18237-1-AP	ProteinTech	Rabbit	Hu, Ms, Rt	http://www.ptglab.com/Products/MMP28-Antibody-18237-1-AP.htm
Mouse Isotype	PA0996	Leica	Mouse		http://www.leica-microsystems.com/fileadmin/img_uploads/novocastra_reagents/Novocastra_datasheets/pa0996.pdf
MPO	a0398	Dako	Rabbit	Hu	http://www.dako.com/us/ar38/p105310/prod_products.htm
MPO	RB-373-A1	ThermoScientific	Rabbit	Hu, Ms	http://www.thermoscientific.com/en/product/myeloperoxidase-mpo-rabbit-polyclonal-antibody.html
Muc1	ab80952	Abcam	A. Hamster	Hu, Ms	http://www.abcam.com/muc1-antibody-mh1--same-as-ct2-ab80952.html
Myosin	M8421	Sigma	Mouse	Rb, Hu, Rt, Ms	http://www.sigmaaldrich.com/catalog/product/sigma/m8421?lang=en&region=US
N-Cadherin	ab18206	Abcam	Rabbit	Ms, Rt, Hu	http://www.abcam.com/n-cadherin-antibody-ab18203.html
NF-KappaB	1546-1	Epitmoics	Rabbit	Ms, Hu	http://www.epitomics.com/products/product_info/298/NF-&%23954%3bB-antibody-1546-1.html
Notch 1	3608	Cell Signaling	Rabbit	Ms, HU, Rt	http://www.cellsignal.com/products/primary-antibodies/notch1-d1e11-xp-rabbit-mab/3608

Pancreatic Polypeptide (PP)	NB100-1793	Novus	Goat	Hu, Ms	http://www.novusbio.com/Pancreatic-Polypeptide-PP-Antibody_NB100-1793.html
PDGFRb	04-397	Millipore	Rabbit	Hu, Ms, Rt	http://www.millipore.com/catalogue/item/04-397
PDL1 (CD276)	ab80276	Abcam	Rat	Ms	http://www.abcam.com/pd-l1-antibody-mih6-low-endotoxin-azide-free-ab80276.html
PDL1 (CD276)	ABC324	Millipore	Rabbit	Hu	http://www.emdmillipore.com/US/en/product/Anti-PDL-1-Antibody,MM_NF-ABC324
PLA2R1	HPA012657	Sigma	Rabbit	Hu	http://www.sigmaaldrich.com/catalog/product/sigma/hpa012657?lang=en&region=US
PPAR	600-401-421	Rockland Inc.	Rabbit	Ms, Rt	http://www.rockland-inc.com/store/Transcription-Factor-Antibodies-600-401-421-O4L_23035.aspx
proSP-C	AB3786	Millipore	Rabbit	Rt, Hu, Ms	http://www.millipore.com/catalogue/item/ab3786
pSTAT	S2690	Sigma	Rabbit	Hu, Ms, Rt	http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/5/s2690dat.Par.0001.File.tmp/s2690dat.pdf
pSTAT3	9145	Cell Signaling	Rabbit	Hu, Ms, Rt	http://www.cellsignal.com/products/9145.html
pSTAT5	9314	Cell Signaling	Rabbit	Hu, Ms	http://www.cellsignal.com/products/9314.html

Rabbit Isotype	AB-105-C	R&D Systems	Rabbit		http://www.rndsystems.com/product_results.aspx?k=AB-105-c
Rabbit Secondary	A16110	Life Technologies	Goat	Rabbit	https://www.lifetechnologies.com/order/catalog/product/A16110?ICID=search-a16110
Rat Isotype	553986	BD Pharmingen			http://www.bdbiosciences.com/ptProduct.jsp?prodId=3268
ROR1	AF2000	R&D Systems	Goat	Hu, Ms	http://www.rndsystems.com/Products/af2000/
S-100	18-0046	Invitrogen	Rabbit	Hu, Ms, Rt	http://www.lifetechnologies.com/order/catalog/product/180046?ICID=search-180046
Siglec-H	NBP2-27062	Novus	Rat	Ms	http://www.novusbio.com/Siglec-H-Antibody-23M15C8_NBP2-27062.html
Smooth Muscle Actin	M0851	Dako	Mouse	Hu, Ms	http://www.dako.com/us/ar38/p102030/prod_products.htm
Smooth Muscle Actin	ab32575	Abcam	Rabbit	Hu, Ms, Rt	http://www.abcam.com/alpha-smooth-muscle-actin-antibody-e184-ab32575.html
Somatostatin	sc-7819	Santa Cruz Biotechnology	Goat	Hu, Rt	http://datasheets.scbt.com/sc-7819.pdf
SV40	554149	BD Pharmingen	Mouse	Ms	http://www.bdbiosciences.com/ptProduct.jsp?prodId=12526&key=554149&param=search&mterms=true&from=dTable
Tyrosine Hydroxylase	MAB318	Millipore	Mouse	Hu, Ms	http://www.millipore.com/catalogue/item/mab318
UPC1	ab23841	Abcam	Rabbit	Ms, Rt	http://www.abcam.com/ucp1-antibody-ab23841.html
Versican	ab177480	Abcam	Rabbit	Ms,Rt,Hu
Vimentin	M0725	Dako	Mouse	Hu	http://www.dako.com/us/ar38/p106290/prod_products.htm
WGA	W11261	Life Technology			http://products.invitrogen.com/ivgn/product/W11261
WNT5A	MA5-15511	ThermoScientific	Mouse	Hu	https://www.fishersci.com/ecomm/servlet/itemdetail?storeId=10652&langId=-1&catalogId=29104&productId=14233375&distype=0&highlightProductsItemsFlag=Y&fromSearch=1&searchType=PROD&hasPromo=0
WNV	W1015	VRL	Rabbit	Ms
WT1	APPB0991	ThermoScientific	Rabbit	Hu, Ms, Rt	http://www.fishersci.com/ecomm/servlet/itemdetail?catnum=APPB0991&storeId=10652
WT1	M356	Dako	Mouse	Hu	http://www.dako.com/us/download.pdf?objectid=106952002

YM1	AF2446	R&D Systems	Goat	Ms	http://www.rndsystems.com/pdf/AF2446.pdf